Journal: Discover Oncology
Article Title: Comprehensive analysis of PANoptosis-related molecular subtypes and prognostic model development of clear cell renal cell carcinoma
doi: 10.1007/s12672-026-04561-9
Figure Lengend Snippet: Knockdown of RBCK1 significantly inhibits ccRCC cell proliferation and migration. A Differential expression analysis of RBCK1 between normal and tumor groups. B–D DSS, PFI, and OS survival curves for low- and high-RBCK1 expression subgroups. E Protein expression levels of RBCK1 in normal and tumor groups based on the CPTAC database. F Protein expression levels of RBCK1 in HK2 cells and 786-O cells with quantitative analysis (n = 3). G Protein expression levels of RBCK1 in HK2 cells and 769-P cells with quantitative analysis (n = 3). H, I Colony formation assay and Transwell migration assay in 786-O cells (n = 3). J, K Colony formation assay and Transwell migration assay in 769-P cells (n = 3). L, M CCK-8 assay showing cell viability at different time points in 786-O and 769-P cells (n = 3). Data are presented as mean ± SD; significance: * P < 0.05, ** P < 0.01, *** P < 0.001
Article Snippet: Human renal proximal tubular epithelial cells HK2, human ccRCC 786-O cells, and human ccRCC 769-P cells were obtained from the American Type Culture Collection (ATCC, USA).
Techniques: Knockdown, Migration, Quantitative Proteomics, Expressing, Colony Assay, Transwell Migration Assay, CCK-8 Assay